library(magrittr)
library(tidyverse)
library(Seurat)
library(readxl)
library(cowplot)
library(colorblindr)
library(viridis)
library(progeny)
theme_cowplot2 <- function(...) {
theme_cowplot(font_size = 12, ...) %+replace%
theme(strip.background = element_blank(),
plot.background = element_blank())
}
theme_set(theme_cowplot2())
coi <- params$cell_type
cell_sort <- params$cell_sort
cell_type_major <- params$cell_type_major
louvain_resolution <- params$louvain_resolution
### load all data ---------------------------------
helper_f <- function(x) ifelse(is.na(x), "", x)
markers_v6 <- yaml::read_yaml("/home/uhlitzf/spectrum_tme/_data/small/signatures/hgsc_v6_major.yaml")
helper_f2 <- function(x) select(unnest(enframe(x, "subtype", "gene"), cols = gene), gene, subtype)
markers_v6_super <- lapply(yaml::read_yaml("/home/uhlitzf/spectrum_tme/_data/small/signatures/hgsc_v6_super.yaml"), helper_f2)
clrs <- yaml::read_yaml("/home/uhlitzf/spectrum_tme/_data/small/signatures/hgsc_v6_colors.yaml") %>%
lapply(function(x) map_depth(x, vec_depth(x)-2, unlist))
names(clrs$patient_id) <- str_remove_all(names(clrs$patient_id), "SPECTRUM-OV-")
meta_tbl <- read_excel("_data/small/MSK SPECTRUM - Single cell RNA-seq_v6.xlsx", sheet = 2) %>%
mutate(patient_id = str_remove_all(patient_id, "SPECTRUM-OV-")) %>%
filter(therapy == "pre-Rx")
signature_tbl <- read_tsv("_data/small/mutational_signatures_summary.tsv") %>%
mutate(patient_id = str_remove_all(patient_id, "SPECTRUM-OV-")) %>%
select(patient_id, consensus_signature) %>%
bind_rows(tibble(patient_id = unique(sort(meta_tbl$patient_id[!(meta_tbl$patient_id %in% .$patient_id)])), consensus_signature = "NA")) %>%
mutate(consensus_signature = ordered(consensus_signature, levels = names(clrs$consensus_signature))) %>%
arrange(consensus_signature)
seu_obj <- read_rds("/work/shah/uhlitzf/data/SPECTRUM/freeze/v5/Ovarian.cancer.cell_highqc.rds")
myfeatures <- c("UMAP_1", "UMAP_2", "umapharmony_1", "umapharmony_2", "sample", "RNA_snn_res.0.1", "RNA_snn_res.0.2", "RNA_snn_res.0.3", "doublet", "nCount_RNA", "nFeature_RNA", "percent.mt", "doublet_score")
plot_data <- as_tibble(FetchData(seu_obj, myfeatures)) %>%
left_join(select(meta_tbl, sample = isabl_id, patient_id, tumor_supersite, tumor_subsite, sort_parameters, therapy),
by = "sample") %>%
mutate(patient_id = str_remove_all(patient_id, "SPECTRUM-OV-"),
RNA_snn_res.0.1 = as.character(RNA_snn_res.0.1),
RNA_snn_res.0.2 = as.character(RNA_snn_res.0.2),
RNA_snn_res.0.3 = as.character(RNA_snn_res.0.3),
tumor_supersite = ordered(tumor_supersite, levels = names(clrs$tumor_supersite))) %>%
mutate(cell_id = colnames(seu_obj)) %>%
left_join(signature_tbl, by = "patient_id")
patient_id <- sort(unique(plot_data$patient_id))
WFDC2, CD24, CLDN3, KRT7, KRT8, KRT17, KRT18, KRT19, EPCAM, WT1, CLDN4, MSLN, FOLR1, MUC1
markers_v6_super[[coi]] %>%
group_by(subtype) %>%
mutate(rank = row_number(gene)) %>%
spread(subtype, gene) %>%
mutate_all(.funs = helper_f) %>%
formattable::formattable()
| rank | Ciliated.cell.1 | Ciliated.cell.2 | Cycling.cancer.cell.1 | Cycling.cancer.cell.2 | doublet.Other | Endometrial.cell | Secretory.cell.1 | Secretory.cell.2 | Secretory.cell.3 | Secretory.cell.4 | Secretory.cell.5 | Secretory.cell.6 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | C20orf85 | KRT14 | CENPF | ATP5MC1 | AGR2 | HP | KRT7 | AGR2 | FBXO32 | AKAP12 | CLDN1 | CXCL10 |
| 2 | CAPS | KRT16 | HMGB2 | PHB | CRISP3 | OBP2A | PLCG2 | CRISP3 | GAS6 | DCDC2 | CLDN16 | IFIT1 |
| 3 | CAPSL | KRT5 | MKI67 | SNF8 | IGFBP7 | PAEP | SCGB2A1 | OVGP1 | MTSS1 | MMP7 | EREG | IFIT2 |
| 4 | PIFO | MMP7 | PTTG1 | SNRPD1 | ITM2B | PTGDS | SLPI | SCGB1A1 | SAMHD1 | TSPAN1 | NDUFB5 | IFIT3 |
| 5 | TPPP3 | SLURP1 | TOP2A | TMEM106C | SPARCL1 | SERINC2 | SST | SCGB3A1 | TFF3 | TSPAN18 | ISG15 | |
| 6 | TFF3 | TFF3 | MX1 |
marker_tbl <- read_tsv("/work/shah/uhlitzf/data/SPECTRUM/freeze/v5/Ovarian.cancer.cell_highqc_markers_02.tsv")
## Hypergeometric test --------------------------------------
test_set <- marker_tbl %>%
group_by(cluster) %>%
filter(!str_detect(gene, "^RPS|^RPL")) %>%
slice(1:50) %>%
mutate(k = length(cluster)) %>%
ungroup %>%
select(cluster, gene, k) %>%
mutate(join_helper = 1) %>%
group_by(cluster, join_helper, k) %>%
nest(test_set = gene)
markers_doub_tbl <- markers_v6 %>%
enframe("subtype", "gene") %>%
filter(!(subtype %in% unique(c(coi, cell_type_major)))) %>%
unnest(gene) %>%
group_by(gene) %>%
filter(length(gene) == 1) %>%
mutate(subtype = paste0("doublet.", subtype)) %>%
bind_rows(tibble(subtype = "Mito.high", gene = grep("^MT-", rownames(seu_obj), value = T)))
ref_set <- markers_v6_super[[coi]] %>%
bind_rows(markers_doub_tbl) %>%
group_by(subtype) %>%
mutate(m = length(gene),
n = length(rownames(seu_obj))-m,
join_helper = 1) %>%
group_by(subtype, m, n, join_helper) %>%
nest(ref_set = gene)
hyper_tbl <- test_set %>%
left_join(ref_set, by = "join_helper") %>%
group_by(cluster, subtype, m, n, k) %>%
do(q = length(intersect(unlist(.$ref_set), unlist(.$test_set)))) %>%
mutate(pval = 1-phyper(q = q, m = m, n = n, k = k)) %>%
ungroup %>%
mutate(qval = p.adjust(pval, "BH"),
sig = qval < 0.01)
# hyper_tbl %>%
# group_by(subtype) %>%
# filter(any(qval < 0.01)) %>%
# ggplot(aes(subtype, -log10(qval), fill = sig)) +
# geom_bar(stat = "identity") +
# facet_wrap(~cluster) +
# coord_flip()
low_rank <- str_detect(unique(hyper_tbl$subtype), "doublet")
subtype_lvl <- c(sort(unique(hyper_tbl$subtype)[!low_rank]), sort(unique(hyper_tbl$subtype)[low_rank]))
cluster_label_tbl <- hyper_tbl %>%
mutate(subtype = ordered(subtype, levels = subtype_lvl)) %>%
arrange(qval, subtype) %>%
group_by(cluster) %>%
slice(1) %>%
mutate(subtype = ifelse(sig, as.character(subtype), paste0("unknown_", cluster))) %>%
select(cluster, cluster_label = subtype) %>%
ungroup %>%
mutate(cluster_label = make.unique(cluster_label, sep = "_"))
seu_obj$cluster_label <- unname(deframe(cluster_label_tbl)[as.character(unlist(seu_obj[[paste0("RNA_snn_res.", louvain_resolution)]]))])
plot_data$cluster_label <- seu_obj$cluster_label
marker_sheet <- marker_tbl %>%
left_join(cluster_label_tbl, by = "cluster") %>%
group_by(cluster_label) %>%
filter(!str_detect(gene, "^RPS|^RPL")) %>%
slice(1:50) %>%
mutate(rank = row_number(-avg_logFC)) %>%
select(cluster_label, gene, rank) %>%
spread(cluster_label, gene) %>%
mutate_all(.funs = helper_f)
formattable::formattable(marker_sheet)
| rank | Ciliated.cell.1 | Ciliated.cell.1_1 | Cycling.cancer.cell.1 | Cycling.cancer.cell.1_1 | Cycling.cancer.cell.2 | doublet.Fibroblast | doublet.Monocyte | doublet.Monocyte_1 | doublet.Other | doublet.Plasma.cell | Endometrial.cell | Secretory.cell.1 | Secretory.cell.1_1 | Secretory.cell.2 | Secretory.cell.2_1 | Secretory.cell.5 | Secretory.cell.6 | unknown_13 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | C20orf85 | CFAP126 | HIST1H4C | CENPF | PHB | TAGLN | IGKC | APOE | CRISP3 | IGLC2 | PAEP | SCGB2A1 | S100A9 | OVGP1 | SPINK1 | CLDN16 | CXCL10 | NTS |
| 2 | CAPS | DNALI1 | TUBA1B | PTTG1 | SNF8 | TPM2 | IGLC2 | NDUFA4L2 | IGFBP7 | IGKC | HP | DAPL1 | SLPI | CRISP3 | CXCL1 | EREG | ISG15 | CALB1 |
| 3 | TPPP3 | PIFO | PCLAF | UBE2S | ATP5MC1 | LGALS1 | IGLC3 | DMKN | ITM2B | CRIP1 | PTGDS | SCGB1D2 | TACSTD2 | PLCG2 | SFTA2 | PCLAF | IFIT3 | MAOB |
| 4 | RSPH1 | FAM183A | ATAD2 | HMGB2 | MEG3 | ACTA2 | IGHG1 | LINC00665 | SCGB1D2 | HIST1H4C | SCGB2A1 | SNHG19 | LCN2 | CCND1 | CXCL8 | HIST1H1A | IFIT1 | SOX18 |
| 5 | C9orf24 | CASC1 | MKI67 | TPX2 | TMBIM4 | CALD1 | SPP1 | COX6B1 | EIF4A2 | IGLC3 | OBP2A | SNHG8 | S100A10 | PTMS | CST6 | RAC3 | MX1 | CDH2 |
| 6 | C5orf49 | RSPH1 | H2AFZ | ASPM | RARRES2 | MT1H | SRGN | GPI | EPB41L4A-AS1 | TOP2A | PCP4 | KCNQ1OT1 | IGFBP3 | SNHG9 | CXCL3 | CTHRC1 | IFIT2 | CHST1 |
| 7 | PIFO | LRRIQ1 | PCNA | MKI67 | UBE2Z | NMU | C1QB | C19orf53 | CIRBP | ATP1B3 | S100A4 | GABPB1-AS1 | GPRC5A | DSTN | CAMK2N1 | TMEM100 | PARP14 | MFSD3 |
| 8 | TMEM190 | C20orf85 | TK1 | CDC20 | PDK2 | RAB32 | CCL4 | PEPD | ASRGL1 | DEFB1 | SERINC2 | KRT17 | PEG10 | SPP1 | MMP23B | IFI6 | RSPO3 | |
| 9 | FAM183A | DNAAF1 | TUBB | TOP2A | ITM2C | SELENOP | GNLY | PSENEN | PIK3R1 | SNRPF | CRABP1 | KRT7 | YWHAE | UPK1B | RRM2 | IFI44L | PIR | |
| 10 | C1orf194 | C5orf49 | CKS1B | CKS2 | SLC35B1 | ANXA3 | RGS1 | PDCD5 | EEF1A1 | HMGN1 | FOLR1 | RARRES1 | APOLD1 | AGR2 | SYT10 | GBP4 | AL589182.1 | |
| 11 | CETN2 | C9orf24 | TYMS | CCNB1 | PCP4 | MT1G | IGHG3 | USP11 | SNHG7 | KLK5 | SCGB1D1 | KRT19 | CDC20 | CAV1 | PHOX2A | STAT1 | MHENCR | |
| 12 | AGR3 | AGR3 | DUT | BIRC5 | XPOT | CDKN2A | C1QA | TMEM147 | NPM1 | IGHG1 | HLA-DQA1 | VEGFA | EPB41L2 | CXCL5 | CNIH2 | TNFSF10 | AP000527.1 | |
| 13 | TXN | C1orf194 | HMGB2 | ARL6IP1 | ZCRB1 | CCT5 | LYZ | IGF2.1 | TPT1 | NNMT | HLA-DRB5 | SOX4 | MYL12B | GNG11 | C1QTNF12 | GBP1 | SIX1 | |
| 14 | ODF3B | ARMC3 | TOP2A | LGALS1 | SPOP | VIM | CCL3 | FBXO2 | BTF3 | IGF1 | SNCG | MMP7 | PTPRA | ADM | TMEM178A | XAF1 | PRRX2 | |
| 15 | CAPSL | CFAP45 | UBE2C | UBE2C | ZNF652 | YBX1 | KRT6A | NPW | FAU | ATP5ME | HLA-DQB1 | NDRG1 | RBMX | AC092069.1 | AC008771.1 | SOD2 | PREX2 | |
| 16 | CFAP126 | EFCAB1 | SMC4 | JPT1 | HOXB4 | AKAP12 | IGFL2-AS1 | CLDN9 | EHF | HMGB1 | AC007906.2 | S100A6 | PABPC4 | CXCL2 | MTRNR2L1 | IDO1 | PYCR3 | |
| 17 | TUBA1A | TPPP3 | DEK | CEP55 | HOXB2 | COTL1 | TYROBP | CPVL | KCNMA1 | PCSK1N | HSPA1A | FTL | METAP2 | PTGS2 | CENPM | OAS1 | FHL1 | |
| 18 | MORN2 | ZMYND10 | MCM3 | CCNB2 | FXYD1 | IGFBP2 | FCER1G | MFAP4 | CAPS | C4orf48 | CHI3L1 | CRYAB | VIM | TNFRSF11B | CENPH | IFI27 | MPPED2 | |
| 19 | C11orf88 | PPIL6 | HELLS | PTMS | TWF1 | FHL2 | PTPRC | GSTM3 | AR | RARRES1 | CYP4B1 | ANXA1 | MYL6B | RHOBTB3 | CENPK | TNFAIP2 | F3 | |
| 20 | DNAAF1 | ENKUR | STMN1 | NMU | C2orf40 | NDUFS6 | FCGR3A | HMGA2 | UGT2B17 | ATP5PF | HLA-DRA | SLC2A1 | RBM8A | VGLL3 | PRTFDC1 | RSAD2 | ACSM3 | |
| 21 | TUBB4B | CDC20B | CDK1 | CDKN3 | KAT7 | PCDH10 | CCL4L2 | FXYD1 | GYPC | HIST1H1C | DEFB1 | TFPI2 | SNHG19 | MALL | POMC | IFIH1 | MMD | |
| 22 | FOXJ1 | SPATA17 | NUSAP1 | TUBA1B | AC008124.1 | EEF1B2 | CCL5 | GFRA1 | EEF2 | S100A10 | HLA-B | FTH1 | WBP11 | EFEMP1 | GPT | PSMB9 | NDP | |
| 23 | ZMYND10 | CAPSL | MCM7 | NUSAP1 | COMP | UCHL1 | LAPTM5 | ZFP14 | EIF1 | DEK | S100A1 | CD55 | PPT1 | WNT2 | MELTF-AS1 | IL32 | AP001528.2 | |
| 24 | LRRIQ1 | TEKT2 | CENPF | H2AFZ | LHX1 | MYH9 | C1QC | HIST1H2BC | ERP27 | UBE2S | HLA-DPA1 | C19orf33 | BRD9 | KRT13 | SPC24 | CXCL11 | APOO | |
| 25 | IGFBP7 | CFAP44 | CLSPN | KPNA2 | SEMA3E | MYL12A | IL7R | COL9A3 | TMEM98 | MT1E | CITED4 | S100A11 | ST13 | IL20RA | MYBL2 | DDX58 | GBP5 | |
| 26 | IK | TEKT1 | MCM4 | HMGB1 | CRTAC1 | SUB1 | ITGB2 | MXRA8 | SORBS2 | NUSAP1 | NPDC1 | TNFRSF12A | SNHG25 | ANKRD1 | OXTR | SAMD9 | RHBDD3 | |
| 27 | EFCAB1 | CFAP54 | UBE2T | HMGN2 | FBXO2 | ANXA5 | CD52 | TIMP3 | HOXB4 | MKI67 | PRSS33 | EMP1 | AMD1 | LINC01127 | AC025159.1 | RNF213 | SAMD10 | |
| 28 | DYDC2 | CFAP43 | MYBL2 | ECT2 | LEMD3 | MYL9 | IGHGP | BGN | PART1 | CCL4 | GMPR | PI3 | SNRPB | CXCL6 | AFAP1-AS1 | IRF1 | NRARP | |
| 29 | EFHC1 | SPEF1 | SMC2 | ANP32E | HOXB-AS1 | CLDN7 | CD14 | IGF2BP3 | FYB2 | SMC1B | HLA-DRB1 | ANXA2 | NAP1L1 | RAB42 | ASF1B | WARS | LINC02303 | |
| 30 | DNALI1 | FAM81B | HMGB1 | CENPE | LGR5 | S100A10 | AIF1 | CA9 | FRMD4A | IGHG3 | GADD45B | MAL2 | SNRPB2 | GABRP | PTPRS | CCL20 | SLC28A2 | |
| 31 | AL357093.2 | EFCAB10 | RAD51AP1 | STMN1 | LMO3 | CD151 | FYB1 | CPS1 | SYNE1 | RGS1 | MESP1 | S100A2 | PA2G4 | XAGE2 | CALML5 | B2M | SOX2 | |
| 32 | C9orf116 | CFAP46 | TPX2 | HMMR | IGF2BP1 | MT1M | CCL3L1 | FBN2 | ALDH1A1 | PTMA | FAM107A | TAGLN | NSA2 | C5orf46 | LINC01436 | CXCL8 | ANKRD6 | |
| 33 | CEP126 | ROPN1L | ZWINT | HMGB3 | IL11RA | CD47 | NKG7 | STC2 | PDE4D | C1QB | MFGE8 | CAMK2N1 | NMT1 | ABLIM3 | HOMER1 | ISG20 | MAN1B1-DT | |
| 34 | SNTN | CFAP73 | RRM2 | TROAP | COL9A3 | FLNA | ALOX5AP | PLOD1 | HSPB6 | SLC16A8 | CYBA | LDHA | TMCO1 | COL4A4 | F2R | TAP1 | KCNMB4 | |
| 35 | CCDC170 | C7orf57 | NASP | KIF20B | MDM1 | IL32 | SAMSN1 | NRN1 | HOXB6 | SRGN | IRX3 | AKAP12 | WDR70 | COL8A1 | SEMA6A | LAP3 | TBX2 | |
| 36 | PERP | SPAG8 | CENPW | SMC4 | TSPOAP1 | MYL12B | CD2 | PAGE2 | KAZALD1 | APOC1 | MYL9 | TGM2 | IGFBP3 | GDF7 | PTGER1 | OAS3 | ABO | |
| 37 | ROPN1L | WDR38 | CENPX | CKS1B | CLDN9 | PSMB6 | IGHG4 | HCST | SYT10 | FAM111B | PRDX5 | ITGB8 | TBCA | CCL20 | FZD8 | HLA-B | BMPR1B-DT | |
| 38 | CKB | WDR49 | TMPO | NUCKS1 | PRELP | ACTR3 | GPR183 | HOXA10-AS | TGFB2 | AIF1 | NUPR1 | BNIP3 | KRT81 | AC245041.2 | HACD1 | IFI16 | FES | |
| 39 | TEKT2 | ZBBX | E2F1 | H2AFV | C12orf56 | PDCD6 | LSP1 | FGFR3 | SH3BP5 | IGHG4 | CLDN3 | SLC40A1 | TTYH1 | BMP4 | SALL4 | LY6E | SLC8A1 | |
| 40 | C9orf135 | DRC1 | RANBP1 | RAD21 | AC090673.1 | SRI | CD3D | CPXM2 | MAP3K1 | TYROBP | SLC26A2 | SQSTM1 | HMGB1 | ALPP | CENPN | PLSCR1 | AL713998.1 | |
| 41 | SPA17 | WDR63 | DNMT1 | NCAPD2 | FXYD6 | NDUFS5 | CD37 | HOTAIRM1 | AKR1C3 | CCL5 | HOMER3 | CD24 | ATP5ME | CDA | SOX15 | HLA-A | ZNF252P-AS1 | |
| 42 | GDF15 | CFAP157 | MAD2L1 | DLGAP5 | FAM117A | COX4I1 | IL1B | NXPH4 | DPP4 | TCHH | HLA-DMA | EZR | AGR3 | MARCH10 | CDC6 | NFKBIA | AC064807.1 | |
| 43 | TEKT1 | DYNLRB2 | TMEM106C | AURKA | CCDC170 | EEF1D | GMFG | KRBOX1 | ACSM3 | C1QA | MGLL | FAM3C | TMA7 | GPR87 | FANCD2 | HLA-C | CPED1 | |
| 44 | PSENEN | C6orf118 | HIST1H1D | CENPW | COL6A2 | TPM3 | MS4A7 | LRIG3 | SLITRK5 | FCGR3A | HLA-DPB1 | MUC4 | SFRP1 | DKK1 | CCR10 | IFI44 | THAP7-AS1 | |
| 45 | WDR38 | RSPH4A | ASPM | NEK2 | CYYR1 | UQCRH | CD7 | SDC2 | PREX2 | DDIT4L | ETV4 | ATP1A1 | TPT1 | SLC16A12 | CACNG4 | OAS2 | UFSP1 | |
| 46 | LRRC23 | DNAH12 | FANCI | TUBB4B | CRYGD | CXCL12 | MS4A6A | TMEM147-AS1 | CMBL | FLG | ATP6V0B | CLDN1 | ANO1 | SULT1E1 | MND1 | BIRC3 | EFHC2 | |
| 47 | ARMC3 | TTLL9 | BIRC5 | PLK1 | GPC6 | PDCL2 | LST1 | AEBP1 | CCND2 | CD2 | UCP2 | P4HA1 | MEG3 | THBD | CASC10 | HERC5 | SYT12 | |
| 48 | MLF1 | MAP3K19 | ORC6 | CENPA | FGF17 | NPTX1 | GZMB | FGF11 | CCDC170 | PTPRC | SPDEF | CST6 | CCND2 | MSX2 | BHMT2 | UBE2L6 | TBX3 | |
| 49 | ERICH3 | MORN5 | DTYMK | MAD2L1 | PLXNA4 | MUC12 | GZMA | KCNG1 | IL18 | IGHGP | JUND | SLC16A3 | CD177 | GATA3 | AC144450.1 | SAMD9L | TMEM135 | |
| 50 | CFAP45 | STMND1 | TUBB4B | TUBA1C | TBX2-AS1 | HPD | CD53 | TGFB1 | SKAP1 | TRBC1 | EMP2 | NEAT1 | PEG3 | CYP2W1 | CGAS | CXCL2 | AL161669.3 |
write_tsv(marker_sheet, paste0("/work/shah/uhlitzf/data/SPECTRUM/freeze/v6/", coi, "_marker_sheet.tsv"))
enframe(sort(table(seu_obj$cluster_label))) %>%
mutate(name = ordered(name, levels = rev(name))) %>%
ggplot() +
geom_bar(aes(name, value), stat = "identity") +
theme(axis.text.x = element_text(angle = 90, hjust = 1, vjust = 0.5)) +
labs(y = c("#cells"), x = "cluster")
alpha_lvl <- ifelse(nrow(plot_data) < 20000, 0.2, 0.1)
pt_size <- ifelse(nrow(plot_data) < 20000, 0.2, 0.05)
common_layers_disc <- list(
geom_point(size = pt_size, alpha = alpha_lvl),
NoAxes(),
guides(color = guide_legend(override.aes = list(size = 2, alpha = 1))),
labs(color = "")
)
common_layers_cont <- list(
geom_point(size = pt_size, alpha = alpha_lvl),
NoAxes(),
scale_color_gradientn(colors = viridis(9)),
guides(color = guide_colorbar())
)
ggplot(plot_data, aes(umapharmony_1, umapharmony_2, color = cluster_label)) +
common_layers_disc +
#facet_wrap(~therapy) +
ggtitle("Sub cluster")
my_subtypes <- names(clrs$cluster_label[[coi]])
my_subtypes <- c(my_subtypes, unlist(lapply(paste0("_", 1:3), function(x) paste0(my_subtypes, x)))) %>% .[!str_detect(., "doublet")]
cells_to_keep <- colnames(seu_obj)[seu_obj$cluster_label %in% my_subtypes]
# seu_obj_sub <- subset(seu_obj, cells = cells_to_keep)
# seu_obj_sub <- RunUMAP(seu_obj_sub, dims = 1:20, reduction = "harmony", reduction.name = "umapharmony", reduction.key = "umapharmony_")
# seu_obj_sub$cluster_label <- seu_obj$cluster_label[colnames(seu_obj) %in% colnames(seu_obj_sub)]
# write_rds(seu_obj_sub, paste0("/work/shah/uhlitzf/data/SPECTRUM/freeze/v6/", coi, "_processed_filtered.rds"))
seu_obj_sub <- read_rds(paste0("/work/shah/uhlitzf/data/SPECTRUM/freeze/v6/", coi, "_processed_filtered.rds"))
plot_data_sub <- as_tibble(FetchData(seu_obj_sub, c(myfeatures, "cluster_label"))) %>%
left_join(select(meta_tbl, sample = isabl_id, patient_id, tumor_supersite, tumor_subsite, sort_parameters, therapy),
by = "sample") %>%
mutate(patient_id = str_remove_all(patient_id, "SPECTRUM-OV-"),
tumor_supersite = ordered(tumor_supersite, levels = names(clrs$tumor_supersite))) %>%
mutate(cell_id = colnames(seu_obj_sub),
cluster_label = ordered(cluster_label, levels = my_subtypes),
) %>%
left_join(signature_tbl, by = "patient_id")
if (cell_sort == "CD45+") {
plot_data_sub <- filter(plot_data_sub, sort_parameters != "singlet, live, CD45-", !is.na(tumor_supersite))
}
if (cell_sort == "CD45-") {
plot_data_sub <- filter(plot_data_sub, sort_parameters != "singlet, live, CD45+", !is.na(tumor_supersite))
}
ggplot(plot_data_sub, aes(umapharmony_1, umapharmony_2, color = cluster_label)) +
common_layers_disc +
# scale_color_manual(values = clrs$cluster_label[[coi]])
#facet_wrap(~cluster_label) +
ggtitle("Sub cluster")
ggplot(plot_data_sub, aes(umapharmony_1, umapharmony_2, color = patient_id)) +
common_layers_disc +
ggtitle("Patient") +
# facet_wrap(~therapy) +
scale_color_manual(values = clrs$patient_id)
ggplot(plot_data_sub, aes(umapharmony_1, umapharmony_2, color = tumor_supersite)) +
# geom_point(aes(umapharmony_1, umapharmony_2),
# color = "grey90", size = 0.01,
# data = select(plot_data_sub, -tumor_supersite)) +
common_layers_disc +
ggtitle("Site") +
# facet_wrap(~therapy) +
scale_color_manual(values = clrs$tumor_supersite)
write_tsv(select(plot_data_sub, cell_id, everything(), -UMAP_1, -UMAP_2, -contains("RNA_")), paste0("/work/shah/uhlitzf/data/SPECTRUM/freeze/v6/", coi, "_embedding.tsv"))
signature_modules <- read_excel("_data/small/signatures/SPECTRUM v5 sub cluster markers.xlsx", sheet = 2, skip = 1, range = "M2:P100") %>%
gather(module, gene) %>%
na.omit() %>%
group_by(module) %>%
do(gene = c(.$gene)) %>%
{setNames(.$gene, .$module)}
signature_modules$ISG.module <- c("CCL5", "CXCL10", "IFNA1", "IFNB1", "ISG15", "IFI27L2", "SAMD9L")
## compute expression module scores
for (i in 1:length(signature_modules)) {
seu_obj_sub <- AddModuleScore(seu_obj_sub, features = signature_modules[i], name = names(signature_modules)[i])
seu_obj_sub[[names(signature_modules)[i]]] <- seu_obj_sub[[paste0(names(signature_modules)[i], "1")]]
seu_obj_sub[[paste0(names(signature_modules)[i], "1")]] <- NULL
print(paste(names(signature_modules)[i], "DONE"))
}
## [1] "CD8.Cytotoxic DONE"
## [1] "CD8.Dysfunctional DONE"
## [1] "CD8.Naive DONE"
## [1] "CD8.Predysfunctional DONE"
## [1] "ISG.module DONE"
## compute progeny scores
progeny_list <- seu_obj_sub@assays$RNA@data[VariableFeatures(seu_obj_sub),] %>%
as.matrix %>%
progeny %>%
as.data.frame %>%
as.list
names(progeny_list) <- make.names(paste0(names(progeny_list), ".pathway"))
for (i in 1:length(progeny_list)) {
seu_obj_sub <- AddMetaData(seu_obj_sub, metadata = progeny_list[[i]],
col.name = names(progeny_list)[i])
}
write_rds(seu_obj_sub, paste0("/work/shah/uhlitzf/data/SPECTRUM/freeze/v6/", coi, "_processed_filtered.rds"))
marker_top_tbl <- marker_sheet[,-1] %>%
slice(1:10) %>%
as.list %>%
.[!str_detect(names(.), "doublet")] %>%
enframe("cluster_label_x", "gene") %>%
unnest(gene)
plot_data_markers <- as_tibble(FetchData(seu_obj_sub, c("cluster_label", myfeatures, unique(marker_top_tbl$gene)))) %>%
gather(gene, value, -c(1:(length(myfeatures)+1))) %>%
left_join(select(meta_tbl, sample = isabl_id, patient_id, tumor_supersite, tumor_subsite, sort_parameters, therapy),
by = "sample") %>%
mutate(patient_id = str_remove_all(patient_id, "SPECTRUM-OV-"),
tumor_supersite = ordered(tumor_supersite, levels = names(clrs$tumor_supersite))) %>%
mutate(cluster_label = ordered(cluster_label, levels = my_subtypes)) %>%
group_by(cluster_label, gene) %>%
summarise(value = mean(value, na.rm = T)) %>%
group_by(gene) %>%
mutate(value = scales::rescale(value)) %>%
left_join(marker_top_tbl, by = "gene") %>%
mutate(cluster_label_x = ordered(cluster_label_x, levels = rev(names(clrs$cluster_label[[coi]]))))
ggplot(plot_data_markers) +
geom_tile(aes(gene, cluster_label, fill = value)) +
theme(axis.text.x = element_text(angle = 90, hjust = 1, vjust = 0.5)) +
facet_grid(~cluster_label_x, scales = "free", space = "free") +
scale_fill_gradientn(colors = viridis(9)) +
labs(fill = "Scaled\nexpression") +
theme(aspect.ratio = 1,
axis.line = element_blank(),
axis.ticks = element_blank(),
axis.title = element_blank())
# ggsave(paste0("_fig/002_marker_heatmap_", coi, ".pdf"), width = nrow(marker_top_tbl)/6, height = 5)
comp_site_tbl <- plot_data_sub %>%
filter(!is.na(tumor_supersite)) %>%
group_by(cluster_label, tumor_supersite) %>%
tally %>%
group_by(tumor_supersite) %>%
mutate(nrel = n/sum(n)*100) %>%
ungroup
pnrel_site <- ggplot(comp_site_tbl) +
geom_bar(aes(tumor_supersite, nrel, fill = cluster_label),
stat = "identity", position = position_stack()) +
scale_y_continuous(expand = c(0, 0)) +
theme(axis.text.x = element_text(angle = 90, hjust = 1, vjust = 0.5)) +
# scale_fill_manual(values = clrs$cluster_label[[coi]]) +
labs(fill = "Cluster", y = "Fraction [%]", x = "")
pnabs_site <- ggplot(comp_site_tbl) +
geom_bar(aes(tumor_supersite, n, fill = cluster_label),
stat = "identity", position = position_stack()) +
scale_y_continuous(expand = c(0, 0)) +
theme(axis.text.x = element_text(angle = 90, hjust = 1, vjust = 0.5)) +
# scale_fill_manual(values = clrs$cluster_label[[coi]]) +
labs(fill = "Cluster", y = "# cells", x = "")
plot_grid(pnabs_site, pnrel_site, ncol = 2, align = "h")
# ggsave(paste0("_fig/02_deep_dive_", coi, "_comp_site.pdf"), width = 8, height = 4)
comp_tbl_sample_sort <- plot_data_sub %>%
group_by(tumor_subsite, tumor_supersite, patient_id, consensus_signature, therapy, cluster_label) %>%
tally %>%
group_by(tumor_subsite, tumor_supersite, patient_id, consensus_signature, therapy) %>%
mutate(nrel = n/sum(n)*100,
nsum = sum(n),
log10n = log10(n),
label_supersite = "Site",
label_mutsig = "Signature",
label_therapy = "Rx") %>%
ungroup %>%
arrange(desc(therapy), tumor_supersite) %>%
mutate(tumor_subsite_rx = paste0(tumor_subsite, "_", therapy)) %>%
mutate(tumor_subsite = ordered(tumor_subsite, levels = unique(tumor_subsite)),
tumor_subsite_rx = ordered(tumor_subsite_rx, levels = unique(tumor_subsite_rx))) %>%
arrange(patient_id) %>%
mutate(label_patient_id = ifelse(as.logical(as.numeric(fct_inorder(as.character(patient_id)))%%2), "Patient1", "Patient2"))
sample_id_x_tbl <- plot_data_sub %>%
mutate(sort_short_x = cell_sort) %>%
distinct(patient_id, sort_short_x, tumor_subsite, therapy, sample) %>%
unite(sample_id_x, patient_id, sort_short_x, tumor_subsite, therapy) %>%
arrange(sample_id_x)
comp_tbl_sample_sort %>%
mutate(sort_short_x = cell_sort) %>%
unite(sample_id_x, patient_id, sort_short_x, tumor_subsite_rx) %>%
select(sample_id_x, cluster_label, n, nrel, nsum) %>%
left_join(sample_id_x_tbl, by = "sample_id_x") %>%
write_tsv(paste0("/work/shah/uhlitzf/data/SPECTRUM/freeze/v6/", coi, "_subtype_compositions.tsv"))
ybreaks <- c(500, 1000, 2000, 4000, 6000, 8000, 10000, 15000, 20000)
max_cells_per_sample <- max(comp_tbl_sample_sort$nsum)
ymaxn <- ybreaks[max_cells_per_sample < ybreaks][1]
comp_plot_wrapper <- function(y = "nrel", switch = NULL) {
if (y == "nrel") ylab <- paste0("Fraction\nof cells [%]")
if (y == "n") ylab <- paste0("Number\nof cells")
p <- ggplot(comp_tbl_sample_sort,
aes_string("tumor_subsite_rx", y, fill = "cluster_label")) +
facet_grid(~patient_id, space = "free", scales = "free", switch = switch) +
coord_cartesian(clip = "off") +
# scale_fill_manual(values = clrs$cluster_label[[coi]]) +
theme(axis.text.x = element_blank(),
axis.title.y = element_text(angle = 0, vjust = 0.5, hjust = 0.5,
margin = margin(0, -0.4, 0, 0, unit = "npc")),
axis.ticks.x = element_blank(),
axis.title.x = element_blank(),
axis.line.x = element_blank(),
strip.text.y = element_blank(),
strip.text.x = element_blank(),
strip.background.y = element_blank(),
strip.background.x = element_blank(),
plot.margin = margin(0, 0, 0, 0, "npc")) +
labs(x = "",
y = ylab) +
guides(fill = FALSE)
if (y == "nrel") p <- p +
geom_bar(stat = "identity") +
scale_y_continuous(expand = c(0, 0),
breaks = c(0, 50, 100),
labels = c("0", "50", "100"))
if (y == "n") p <- p +
geom_bar(stat = "identity", position = position_stack()) +
scale_y_continuous(expand = c(0, 0),
breaks = c(0, ymaxn/2, ymaxn),
limits = c(0, ymaxn),
labels = c("", ymaxn/2, ymaxn)) +
expand_limits(y = c(0, ymaxn)) +
theme(panel.grid.major.y = element_line(linetype = 1, color = "grey90", size = 0.5))
return(p)
}
common_label_layers <- list(
geom_tile(color = "white", size = 0.15),
theme(axis.text.x = element_blank(),
axis.ticks = element_blank(),
axis.title.x = element_blank(),
axis.line.x = element_blank(),
strip.text = element_blank(),
strip.background = element_blank(),
plot.margin = margin(0, 0, 0, 0, "npc")),
scale_y_discrete(expand = c(0, 0)),
labs(y = ""),
guides(fill = FALSE),
facet_grid(~patient_id,
space = "free", scales = "free")
)
comp_label_site <- ggplot(distinct(comp_tbl_sample_sort, tumor_subsite_rx, therapy, tumor_supersite, label_supersite, patient_id),
aes(tumor_subsite_rx, label_supersite,
fill = tumor_supersite)) +
scale_fill_manual(values = clrs$tumor_supersite) +
common_label_layers
comp_label_rx <- ggplot(distinct(comp_tbl_sample_sort, tumor_subsite_rx, therapy, tumor_supersite, label_therapy, consensus_signature, patient_id),
aes(tumor_subsite_rx, label_therapy,
fill = therapy)) +
scale_fill_manual(values = c(`post-Rx` = "gold3", `pre-Rx` = "steelblue")) +
common_label_layers
comp_label_mutsig <- ggplot(distinct(comp_tbl_sample_sort, tumor_subsite_rx, therapy, tumor_supersite, label_mutsig, consensus_signature, patient_id),
aes(tumor_subsite_rx, label_mutsig,
fill = consensus_signature)) +
scale_fill_manual(values = clrs$consensus_signature) +
common_label_layers
patient_label_tbl <- distinct(comp_tbl_sample_sort, patient_id, .keep_all = T)
comp_label_patient_id <- ggplot(patient_label_tbl, aes(tumor_subsite_rx, label_patient_id)) +
scale_fill_manual(values = clrs$consensus_signature) +
geom_text(aes(tumor_subsite_rx, label_patient_id, label = patient_id)) +
facet_grid(~patient_id,
space = "free", scales = "free") +
coord_cartesian(clip = "off") +
theme_void() +
theme(strip.text = element_blank(),
strip.background = element_blank(),
plot.margin = margin(0, 0, 0, 0, "npc"))
hist_plot_wrapper <- function(x = "nrel") {
if (x == "nrel") {
xlab <- paste0("Fraction of cells [%]")
bw <- 5
}
if (x == "log10n") {
xlab <- paste0("Number of cells")
bw <- 0.2
}
p <- ggplot(comp_tbl_sample_sort) +
ggridges::geom_density_ridges(
aes_string(x, "cluster_label", fill = "cluster_label"), color = "black",
stat = "binline", binwidth = bw, scale = 3) +
facet_grid(label_supersite~.,
space = "free", scales = "free") +
# scale_fill_manual(values = clrs$cluster_label[[coi]]) +
theme(axis.text.y = element_blank(),
axis.ticks.y = element_blank(),
axis.title.y = element_blank(),
axis.line.y = element_blank(),
strip.text = element_blank(),
strip.background = element_blank(),
plot.margin = margin(0, 0, 0, 0, "npc")) +
scale_x_continuous(expand = c(0, 0)) +
scale_y_discrete(expand = c(0, 0)) +
guides(fill = FALSE) +
labs(x = xlab)
if (x == "log10n") p <- p + expand_limits(x = c(0, 3)) +
scale_x_continuous(expand = c(0, 0),
labels = function(x) parse(text = paste("10^", x)))
return(p)
}
pcomp1 <- comp_plot_wrapper("n")
pcomp2 <- comp_plot_wrapper("nrel")
pcomp_grid <- plot_grid(comp_label_patient_id,
pcomp1, pcomp2,
comp_label_site, comp_label_rx, comp_label_mutsig,
ncol = 1, align = "v",
rel_heights = c(0.15, 0.33, 0.33, 0.06, 0.06, 0.06))
phist1 <- hist_plot_wrapper("log10n")
pcomp_hist_grid <- ggdraw() +
draw_plot(pcomp_grid, x = 0.01, y = 0, width = 0.85, height = 1) +
draw_plot(phist1, x = 0.87, y = 0.05, width = 0.12, height = 0.8)
pcomp_hist_grid
# ggsave(paste0("_fig/02_composition_v6_",coi,".pdf"), pcomp_hist_grid, width = 10, height = 2)
comp_tbl_z <- comp_tbl_sample_sort %>%
filter(therapy == "pre-Rx",
!(tumor_supersite %in% c("Ascites", "Other"))) %>%
group_by(patient_id, cluster_label) %>%
arrange(patient_id, cluster_label, nrel) %>%
mutate(rank = row_number(nrel),
z_rank = scales::rescale(rank)) %>%
mutate(mean_nrel = mean(nrel, na.rm = T),
sd_nrel = sd(nrel, na.rm = T),
z_nrel = (nrel - mean_nrel) / sd_nrel) %>%
ungroup()
ggplot(comp_tbl_z) +
geom_boxplot(aes(tumor_supersite, z_nrel, color = tumor_supersite),
outlier.shape = NA) +
geom_point(aes(tumor_supersite, z_nrel, color = tumor_supersite), position = position_jitter(), size = 0.1) +
facet_grid(~cluster_label, scales = "free_x", space = "free_x") +
theme(axis.text.x = element_text(angle = 90, hjust = 1, vjust = 0.5),
aspect.ratio = 5) +
scale_color_manual(values = clrs$tumor_supersite)
ggplot(comp_tbl_z) +
geom_boxplot(aes(tumor_supersite, z_rank, color = tumor_supersite),
outlier.shape = NA) +
geom_point(aes(tumor_supersite, z_rank, color = tumor_supersite), position = position_jitter(), size = 0.1) +
facet_grid(~cluster_label, scales = "free_x", space = "free_x") +
theme(axis.text.x = element_text(angle = 90, hjust = 1, vjust = 0.5),
aspect.ratio = 5) +
scale_color_manual(values = clrs$tumor_supersite)
devtools::session_info()
## ─ Session info ───────────────────────────────────────────────────────────────
## setting value
## version R version 3.6.2 (2019-12-12)
## os Debian GNU/Linux 10 (buster)
## system x86_64, linux-gnu
## ui X11
## language (EN)
## collate en_US.UTF-8
## ctype en_US.UTF-8
## tz Etc/UTC
## date 2020-12-05
##
## ─ Packages ───────────────────────────────────────────────────────────────────
## package * version date lib
## ape 5.3 2019-03-17 [2]
## assertthat 0.2.1 2019-03-21 [2]
## backports 1.1.10 2020-09-15 [1]
## bibtex 0.4.2.2 2020-01-02 [2]
## Biobase 2.46.0 2019-10-29 [2]
## BiocGenerics 0.32.0 2019-10-29 [2]
## bitops 1.0-6 2013-08-17 [2]
## broom 0.7.2 2020-10-20 [1]
## callr 3.4.2 2020-02-12 [1]
## caTools 1.17.1.4 2020-01-13 [2]
## cellranger 1.1.0 2016-07-27 [2]
## cli 2.0.2 2020-02-28 [1]
## cluster 2.1.0 2019-06-19 [3]
## codetools 0.2-16 2018-12-24 [3]
## colorblindr * 0.1.0 2020-01-13 [2]
## colorspace * 1.4-2 2019-12-29 [2]
## cowplot * 1.0.0 2019-07-11 [2]
## crayon 1.3.4 2017-09-16 [1]
## data.table 1.12.8 2019-12-09 [2]
## DBI 1.1.0 2019-12-15 [2]
## dbplyr 2.0.0 2020-11-03 [1]
## desc 1.2.0 2018-05-01 [2]
## devtools 2.2.1 2019-09-24 [2]
## digest 0.6.25 2020-02-23 [1]
## dplyr * 1.0.2 2020-08-18 [1]
## ellipsis 0.3.1 2020-05-15 [1]
## evaluate 0.14 2019-05-28 [2]
## fansi 0.4.1 2020-01-08 [2]
## farver 2.0.3 2020-01-16 [1]
## fitdistrplus 1.0-14 2019-01-23 [2]
## forcats * 0.5.0 2020-03-01 [1]
## formattable 0.2.0.1 2016-08-05 [1]
## fs 1.5.0 2020-07-31 [1]
## future 1.15.1 2019-11-25 [2]
## future.apply 1.4.0 2020-01-07 [2]
## gbRd 0.4-11 2012-10-01 [2]
## gdata 2.18.0 2017-06-06 [2]
## generics 0.0.2 2018-11-29 [2]
## ggplot2 * 3.3.2 2020-06-19 [1]
## ggrepel 0.8.1 2019-05-07 [2]
## ggridges 0.5.2 2020-01-12 [2]
## globals 0.12.5 2019-12-07 [2]
## glue 1.3.2 2020-03-12 [1]
## gplots 3.0.1.2 2020-01-11 [2]
## gridExtra 2.3 2017-09-09 [2]
## gtable 0.3.0 2019-03-25 [2]
## gtools 3.8.1 2018-06-26 [2]
## haven 2.3.1 2020-06-01 [1]
## hms 0.5.3 2020-01-08 [1]
## htmltools 0.4.0 2019-10-04 [2]
## htmlwidgets 1.5.1 2019-10-08 [2]
## httr 1.4.2 2020-07-20 [1]
## ica 1.0-2 2018-05-24 [2]
## igraph 1.2.5 2020-03-19 [1]
## irlba 2.3.3 2019-02-05 [2]
## jsonlite 1.7.1 2020-09-07 [1]
## KernSmooth 2.23-16 2019-10-15 [3]
## knitr 1.26 2019-11-12 [2]
## labeling 0.3 2014-08-23 [2]
## lattice 0.20-38 2018-11-04 [3]
## lazyeval 0.2.2 2019-03-15 [2]
## leiden 0.3.1 2019-07-23 [2]
## lifecycle 0.2.0 2020-03-06 [1]
## listenv 0.8.0 2019-12-05 [2]
## lmtest 0.9-37 2019-04-30 [2]
## lsei 1.2-0 2017-10-23 [2]
## lubridate 1.7.9.2 2020-11-13 [1]
## magrittr * 2.0.1 2020-11-17 [1]
## MASS 7.3-51.5 2019-12-20 [3]
## Matrix 1.2-18 2019-11-27 [3]
## memoise 1.1.0 2017-04-21 [2]
## metap 1.2 2019-12-08 [2]
## mnormt 1.5-5 2016-10-15 [2]
## modelr 0.1.8 2020-05-19 [1]
## multcomp 1.4-12 2020-01-10 [2]
## multtest 2.42.0 2019-10-29 [2]
## munsell 0.5.0 2018-06-12 [2]
## mutoss 0.1-12 2017-12-04 [2]
## mvtnorm 1.0-12 2020-01-09 [2]
## nlme 3.1-143 2019-12-10 [3]
## npsurv 0.4-0 2017-10-14 [2]
## numDeriv 2016.8-1.1 2019-06-06 [2]
## pbapply 1.4-2 2019-08-31 [2]
## pillar 1.4.6 2020-07-10 [1]
## pkgbuild 1.0.6 2019-10-09 [2]
## pkgconfig 2.0.3 2019-09-22 [1]
## pkgload 1.0.2 2018-10-29 [2]
## plotly 4.9.1 2019-11-07 [2]
## plotrix 3.7-7 2019-12-05 [2]
## plyr 1.8.5 2019-12-10 [2]
## png 0.1-7 2013-12-03 [2]
## prettyunits 1.1.1 2020-01-24 [1]
## processx 3.4.2 2020-02-09 [1]
## progeny * 1.11.3 2020-10-22 [1]
## ps 1.3.2 2020-02-13 [1]
## purrr * 0.3.4 2020-04-17 [1]
## R.methodsS3 1.7.1 2016-02-16 [2]
## R.oo 1.23.0 2019-11-03 [2]
## R.utils 2.9.2 2019-12-08 [2]
## R6 2.4.1 2019-11-12 [1]
## RANN 2.6.1 2019-01-08 [2]
## rappdirs 0.3.1 2016-03-28 [2]
## RColorBrewer 1.1-2 2014-12-07 [2]
## Rcpp 1.0.4 2020-03-17 [1]
## RcppAnnoy 0.0.16 2020-03-08 [1]
## RcppParallel 4.4.4 2019-09-27 [2]
## Rdpack 0.11-1 2019-12-14 [2]
## readr * 1.4.0 2020-10-05 [1]
## readxl * 1.3.1 2019-03-13 [2]
## rematch 1.0.1 2016-04-21 [2]
## remotes 2.1.0 2019-06-24 [2]
## reprex 0.3.0 2019-05-16 [2]
## reshape2 1.4.3 2017-12-11 [2]
## reticulate 1.14 2019-12-17 [2]
## rlang 0.4.8 2020-10-08 [1]
## rmarkdown 2.0 2019-12-12 [2]
## ROCR 1.0-7 2015-03-26 [2]
## rprojroot 1.3-2 2018-01-03 [2]
## rstudioapi 0.11 2020-02-07 [1]
## rsvd 1.0.3 2020-02-17 [1]
## Rtsne 0.15 2018-11-10 [2]
## rvest 0.3.6 2020-07-25 [1]
## sandwich 2.5-1 2019-04-06 [2]
## scales 1.1.0 2019-11-18 [2]
## sctransform 0.2.1 2019-12-17 [2]
## SDMTools 1.1-221.2 2019-11-30 [2]
## sessioninfo 1.1.1 2018-11-05 [2]
## Seurat * 3.1.2 2019-12-12 [2]
## sn 1.5-4 2019-05-14 [2]
## stringi 1.5.3 2020-09-09 [1]
## stringr * 1.4.0 2019-02-10 [1]
## survival 3.1-8 2019-12-03 [3]
## testthat 2.3.2 2020-03-02 [1]
## TFisher 0.2.0 2018-03-21 [2]
## TH.data 1.0-10 2019-01-21 [2]
## tibble * 3.0.4 2020-10-12 [1]
## tidyr * 1.1.2 2020-08-27 [1]
## tidyselect 1.1.0 2020-05-11 [1]
## tidyverse * 1.3.0 2019-11-21 [2]
## tsne 0.1-3 2016-07-15 [2]
## usethis 1.5.1 2019-07-04 [2]
## uwot 0.1.5 2019-12-04 [2]
## vctrs 0.3.5 2020-11-17 [1]
## viridis * 0.5.1 2018-03-29 [2]
## viridisLite * 0.3.0 2018-02-01 [2]
## withr 2.3.0 2020-09-22 [1]
## xfun 0.12 2020-01-13 [2]
## xml2 1.3.2 2020-04-23 [1]
## yaml 2.2.1 2020-02-01 [1]
## zoo 1.8-7 2020-01-10 [2]
## source
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.3)
## CRAN (R 3.6.2)
## Bioconductor
## Bioconductor
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## Github (clauswilke/colorblindr@1ac3d4d)
## R-Forge (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.3)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.3)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.3)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.3)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## Bioconductor
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.3)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## Github (saezlab/progeny@94bea1c)
## CRAN (R 3.6.2)
## CRAN (R 3.6.3)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.3)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.3)
## CRAN (R 3.6.3)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.3)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
## CRAN (R 3.6.2)
##
## [1] /home/uhlitzf/R/lib
## [2] /usr/local/lib/R/site-library
## [3] /usr/local/lib/R/library